Handbook of Immunoassay Technologies: Approaches, Performances, and Applications unravels the role of immunoassays in the biochemical sciences. During the last four decades, a wide range of immunoassays has been developed, ranging from the conventional enzyme-linked immunosorbent assays, to the smartphone-based point-of-care formats. The advances in rapid biochemical procedures, novel biosensing schemes, fully integrated lab-on-a-chip platforms, prolonged biomolecular storage strategies, device miniaturization and interfacing, and emerging smart system technologies equipped with personalized mobile healthcare tools are paving the way to next-generation immunoassays, and are all discussed in this comprehensive text.

Immunoassays play a prominent role in clinical diagnostics as they are the eyes of healthcare professionals, helping them make informed clinical decisions via confirmed disease diagnosis, and thus enabling favorable health outcomes. The faster and reliable diagnosis of infections will further control their spread to uninfected persons. Similarly, immunoassays play a prominent role in veterinary diagnostics, food analysis, environmental monitoring, defense and security, and other bioanalytical settings. Therefore, they enable the detection of a plethora of analytes, which includes disease biomarkers, pathogens, drug impurities, environmental contaminants, allergens, food adulterants, drugs of abuse and various biomolecules.

- Provides a valuable increase of understanding of cellular and biomedical functions

- Gives the most updated resource in the field of immunoassays, providing the comprehensive details of various types of immunoassays that need to be performed in healthcare, and in industrial, environmental and other biochemical settings

- Discusses all multifarious aspects of immunoassays

- Describes the immunoassay formats, along with their principle of operation, characteristics, pros and cons, and potential biochemical and bioanalytical applications

- Provides extensive knowledge and guided insights as detailed by experienced, renowned experts and key opinion makers in the field of immunoassays

Table of Contents

1. lmmunoassays: An Overview

2. Antibody Immobilization and Surface Functionalization Chemistries for lmmunodiagnostics

3. Monoclonal Antibody Generation by Phage Display: History, State-of-the-Art, and Future

4. Bioanalytical Requirements and Regulatory Guidelines for lmmunoassays

5. Enzyme- Linked lmmunoassays

6. Surface Plasmon Resonance-Based lmmunoassays: Approaches, Performance, and Applications

7. Lateral Flow lmmunoassays

8. Paper-Based lmmunoassays

9. Acoustic Wave-Based lmmunoassays

10. Interferometry-Based lmmunoassays

11 . Nanomaterial- and Micromaterial-Based lmmunoassays

12. Microcantilever-Based Sensors

13. Quartz Crystal Microbalance-Based Sensors

14. Electrochemical lmmunosensors: Fundamentals and Applications in Clinical Diagnostics

15. Lab-on-a-Chip (LOC) lmmunoassays

16. Smartphone-Based lmmunoassays

17. lmmunoassays: Future Prospects and Possibilities

Review

Describes the immunoassay formats, their principle of operation, characteristics, pros and cons, and potential biochemical and bioanalytical applications

About the Authors

John H.T. Luong was the Walton Fellow associated with the School of Chemistry of University College Cork, Ireland. He was the Professor and Division Head of Biomedical Engineering at Nanyang Technological University, Singapore. He was also a distinguished Visiting Professor at the University of Zhengzhou (China), a Visiting Professor at Nanocore, National University of Singapore, Bar-Ilan University (Ramat Gan-Israel), Institut d'Electronique, de Microélectronique et de Nanotechnologie, Université de Lille (France), and Delhi Technological University, India. He has received several awards for his excellent contribution to research, innovation, and technology transfer. He is on the Editorial Board of several Journals, the Chief Editor of MDPI Journal of Functional Biomaterials-Antimicrobials, and the Associate Editor of MDPI Macromolecule. He has 10 patents and 350 publications with over 31,000 citations with an h-index of 89. He is the expert/reviewer/advisor/consultant for many international funding agencies and enterprises.

Sandeep K. Vashist is the Senior Global IVD Product Director at Fapon Biotech Inc., Germany. He held senior R&D and management roles in large IVD giants, such as Bristol-Myers Squibb, Revvity (Immunodiagnostic Systems), and Trivitron Healthcare (Labsystems Diagnostics). Besides, he has been the Chief of the Research Advisory Board at Sensing Self Pte Ltd., Singapore, an Advisory Board Member at Calciscon AG, Switzerland, and a Board Member at Antrum Biotech, South Africa. Sandeep invented and pioneered many CE-certified and/or FDA-approved IVD kits, devices, and technologies. He has 12 patents, over 300 publications, 3 books, and over 9000 international citations. He is the Editor and Reviewer of several journals, funding agencies, and international organizations. Sandeep is an IVD veteran with over two decades of rich global IVD experience.