Genomes 5 has been completely revised and updated. It is a thoroughly modern textbook about genomes and how they are investigated. As with previous Genomes editions, techniques come first, then genome anatomies, followed by genome function, and finally genome evolution. The genomes of all types of organism are covered: viruses, bacteria, fungi, plants, and animals, including humans and other hominids.

Genome sequencing and assembly methods have been thoroughly revised to include new developments in long-read DNA sequencing. Coverage of genome annotation emphasizes genome-wide RNA mapping, with CRISPR-Cas 9 and GWAS methods of determining gene function covered. The knowledge gained from these techniques forms the basis of the chapters that describe the three main types of genomes: eukaryotic, prokaryotic (including eukaryotic organelles), and viral (including mobile genetic elements). Coverage of genome expression and replication is truly genomic, concentrating on the genome-wide implications of DNA packaging, epigenome modifications, DNA-binding proteins, non-coding RNAs, regulatory genome sequences, and protein-protein interactions. Also included are examples of the applications of metabolomics and systems biology. The final chapter is on genome evolution, including the evolution of the epigenome, using genomics to study human evolution, and using population genomics to advance plant breeding. Established methods of molecular biology are included if they are still relevant today and there is always an explanation as to why the method is still important.

Genomes 5 is the ideal text for upper-level courses focused on genomes and genomics.

Key Features

• A highly accessible and well-structured book with chapters organized into four parts to aid navigation
• Superb artwork illustrates the key concepts and mechanisms
• Each chapter has a set of short-answer questions and in-depth problems to test the reader’s understanding of the material
• Thoroughly up to date with references to the latest research from the 2020s

Table of Contents

Part 1 How genomes are studied

Chapter 1 Genomes, transcriptomes, and proteomes

Chapter 2 Studying DNA

Chapter 3 Mapping genomes

Chapter 4 Sequencing genomes

Chapter 5 Genome annotation

Chapter 6 Identifying gene functions

Part 2 Genome anatomies

Chapter 7 Eukaryotic nuclear genomes

Chapter 8 Genomes of prokaryotes and eukaryotic organelles

Chapter 9 Virus genomes and mobile genetic elements

Part 3 How genomes are expressed

Chapter 10 Accessing the genome

Chapter 11 The role of DNA-binding proteins in genome expression

Chapter 12 Transcriptomes

Chapter 13 Proteomes

Chapter 14 Genome expression in the context of cell and organism

Part 4 How genomes replicate and evolve

Chapter 15 Genome Replication

Chapter 16 Recombination and transposition

Chapter 17 Mutations and DNA repair

Chapter 18 How genomes evolve

Review

"… a great resource for students wanting to understand genomes and the tremendous advancements in the field …" Ian Gonzales (Yale School of Medicine)

"[The book] does exactly what it is supposed to do, describing the current and ever-expanding filed of genomics. I really liked this book and learned so much from reading it thoroughly. It has certainly updated my own knowledge and I would highly recommend it to all, … I am only disappointed I did not have a book as informative as this when I did my undergraduate study many years ago.” Tony Reynolds (The Biologist)

About the Author

Terry Brown is professor of Biomolecular Archaeology at Manchester University. As well as writing the previous editions of Genomes, he is the author of Introduction to Genetics: A Molecular Approach (Garland 2011) and 5 editions of DNA Cloning Analysis (Wiley Blackwell). He is also an experienced teacher of genetics.