Freshney’s Culture of Animal Cells is the most comprehensive and up-to-date resource on the principles, techniques, equipment, and applications in the field of cell and tissue culture. Explaining both how to do tissue culture and why a technique is done in a particular way, this classic text covers the biology of cultured cells, how to select media and substrates, regulatory requirements, laboratory protocols, aseptic technique, experimental manipulation of animal cells, and much more.

The eighth edition contains extensively revised material that reflects the latest techniques and emerging applications in cell culture, such as the use of CRISPR/Cas9 for gene editing and the adoption of chemically defined conditions for stem cell culture. A brand-new chapter examines the origin and evolution of cell lines, joined by a dedicated chapter on irreproducible research, its causes, and the importance of reproducibility and good cell culture practice. Throughout the book, updated chapters and protocols cover topics including live-cell imaging, 3D culture, scale-up and automation, microfluidics, high-throughput screening, and toxicity testing. This landmark text:

• Provides comprehensive single-volume coverage of basic skills and protocols, specialized techniques and applications, and new and emerging developments in the field
• Covers every essential area of animal cell culture, including lab design, disaster and contingency planning, safety, bioethics, media preparation, primary culture, mycoplasma and authentication testing, cell line characterization and cryopreservation, training, and troubleshooting
• Features a wealth of new content including protocols for gene delivery, iPSC generation and culture, and tumor spheroid formation
• Includes an updated and expanded companion website containing figures, artwork, and supplementary protocols to download and print

The eighth edition of Freshney’s Culture of Animal Cells is an indispensable volume for anyone involved in the field, including undergraduate and graduate students, clinical and biopharmaceutical researchers, bioengineers, academic research scientists, and managers, technicians, and trainees working in cell biology, molecular biology, and genetics laboratories.

Table of Contents

Chapter 1 Introduction

Chapter 2 Biology of Cultured Cells

Chapter 3 Origin and Evolution of Cultured Cells

Chapter 4 Laboratory Design And Layout

Chapter 5 Equipment And Materials

Chapter 6 Safety And Bioethics

Chapter 7 Reproducibility And Good Cell Culture Practice

Chapter 8 Culture Vessels And Substrates

Chapter 9 Defined Media And Supplements

Chapter 10 Serum-Free Media

Chapter 11 Preparation And Sterilization

Chapter 12 Aseptic Technique

Chapter 13 Primary Culture

Chapter 14 Subculture And Cell Lines

Chapter 15 Cryopreservation And Banking

Chapter 16 Microbial Contamination

Chapter 17 Cell Line Misidentification And Authentication

Chapter 18 Cell Line Characterization

Chapter 19 Quantitation And Growth Kinetics

Chapter 20 Cell Cloning And Selection

Chapter 21 Cell Separation And Sorting

Chapter 22 Genetic Modification And lmmortalization

Chapter 23 Culture Of Stem Cells

Chapter 24 Culture Of Specific Cell Types

Chapter 25 Culture Of Tumor Cells

Chapter 26 Differentiation

Chapter 27 Three-Dimensional Culture

Chapter 28 Scale-Up And Automation

Chapter 29 Toxicity Testing

Chapter 30 Training

Chapter 31 Problem Solving

Chapter 32 In Conclusion

Appendix A Glossary

Appendix B Calculations And Preparation Of Reagents

Appendix C Media Formulations

About the Authors

Amanda Capes-Davis, is a cell culture scientist and technical writer. She was Founding Manager and Honorary Scientist at CellBank Australia, Children’s Medical Research Institute (CMRI), and is a member of the International Cell Line Authentication Committee (ICLAC). She was a Reviewing Editor for the 7th edition of Culture of Animal Cells, and has written numerous journal articles, policies, protocols, and white papers on good cell culture practice.

R. Ian Freshney, was an honorary Senior Research Fellow at the Institute of Cancer Sciences at the University of Glasgow, UK. Dr Freshney, who died in 2019, was a world-renowned cancer biologist and a pioneer in cell culture techniques who made important contributions to new approaches for treating cancer patients. He taught cell culture courses at national and international level, and wrote and edited numerous books, including the first seven editions of Culture of Animal Cells.